Protocol biopsies are on days 106 (Cr = 1.2), 182 (Cr = 1.4), 225 (Cr = 1.8) and 352 (Cr. 2.4) and autopsy on day 371 (Cr = 4.8). Hematoxylin-eosin stained images (A–E) and C4d immunohistochemistry and hematoxylin counterstained images (F–J). Alloantibody was detected on day 162, and C4d staining is positive and TG is present on days 225, 352 and 371. Original magnification, 400×.