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. Author manuscript; available in PMC: 2010 Nov 24.
Published in final edited form as: Biochemistry. 2009 Nov 24;48(46):11142–11148. doi: 10.1021/bi901343d

FIGURE 1.

FIGURE 1

LC-MS-ESI+ chromatogram of trypsin-digested Ub (bottom), digest of Ub that was oxidized with 10 % molar excess of H2O2 (middle), and digest of Ub that was oxidized with 10 molar equivalents of HOCl (top). The time scale shown is for native Ub. The other chromatograms were aligned by best-fit of the retention time and fragment m/z values. Based on the observed m/z values, the following modifications of the Met-Gln-Ile-Phe-Val-Lys (1–6) peptide of Ub have been assigned: (A) Met-Gln-Ile-Phe-Val-Lys (retention time ca. 53 min, m/z = 764.4 + H+), (B) MetO-Gln-Ile-Phe-Val-Lys (retention time ca. 46–47 min, m/z = 780.4 + H+), and (C) DHM-Gln-Ile-Phe-Val-Lys (retention time ca. 48 min, m/z = 762.4 + H+).