. Author manuscript; available in PMC: 2010 Oct 13.

Published in final edited form as: Biochemistry. 2009 Oct 13;48(40):9569–9581. doi: 10.1021/bi901182w

Table 1.

Molecular mass analysis of Fra2, Grx3, and Grx4 complexes. All masses shown in Da.

Sample	Gel Filtration	MALDI	Theoretical
Apo-Fra2	19500	13971, 12824^a	13971 (monomer)
Apo-Grx3	37000	28120	28130 (monomer)
Apo-Grx4	37200	27492	27493 (monomer)
[2Fe-2S] Grx3-Fra2	52600	ND^b	42101 (heterodimer)
[2Fe-2S] Grx4-Fra2	52800	ND	41464 (heterodimer)
[2Fe-2S] Grx3	71500	ND	56260 (homodimer)
[2Fe-2S] Grx4	65700	ND	54968 (homodimer)

This second peak is attributed to the Fra2 proteolytic fragment shown in Figure 1A.

ND, not determined