Figure 1.

Injection of PC61 mAb depletes CD4⁺CD25⁺ cells. BALB/c mice (2 animals per group) were injected ip with DPBS (a), 100 μg (b), or 200 μg (c) of purified anti-CD25 mAb (PC61). Two days later, spleen cells were obtained; immunofluorescence was carried out using anti-CD4-FITC and anti-CD25-PE mAbs, and cells were analyzed by FACS. The lymphocyte region was first identified by FSC and SSC characteristics, gated, and 10,000 gated events were captured. Numbers indicate % of CD4⁺CD25⁺ cells within the CD4⁺ population.