Fig. 3.

Effects of PGE₂ on the expressions of E-cadherin and Snail, and cell mobility in HCT8 and HT29. (A) Western blot analyses for E-cadherin and Snail in the presence of PGE₂ at the indicated doses for 24 hours. Forty µg of protein was separated by 10% SDS-polyacrylamide gel electrophoresis and transferred to a nitrocellulose membrane. The bottom represents GAPDH, which was used as a loading control. (B) Cells were incubated with serum-free medium including with PGE₂ (5 µg/mL) and allowed to migrate into the wound area for up to 24 hours at 37℃. Images were acquired immediately, and at 24 hours after wounding. PGE₂ (5 µg/mL) treatment increased cell mobility in HCT8 and HT29. GAPDH, glyceraldehydes 3-phosphate dehydrogenase; PGE₂, prostaglandin E₂. SDS, sodium dodecyl sulfate.