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. Author manuscript; available in PMC: 2009 Dec 21.
Published in final edited form as: J Biol Chem. 2001 Jul 30;276(39):36446–36453. doi: 10.1074/jbc.M104386200

Fig. 4. Gel mobility shift assays of wt and mutant RPA.

Fig. 4

Increasing amounts of wt or the indicated mutant RPA (0, 1, 2, 6, 25, 50, 100, 250, 500, 1000, 2000, 6000, 20000 or 60000 pg) were incubated with 2 fmols of radiolabeled (dT)17 (A) or (dT)40 (B). The reactions were then resolved on a 6% nondenaturing polyacrylamide gel. The first lane of each gel (−), is a negative control reaction containing no protein. The positions of RPA-DNA complexes (S, singly-liganded; M, multiply-liganded) and unbound oligonucleotide (F) are indicated. The asterisk indicates a binding reaction containing equimolar amounts of RPA and substrate.