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. Author manuscript; available in PMC: 2010 Nov 15.
Published in final edited form as: J Neurosci Methods. 2009 Aug 25;184(2):332–336. doi: 10.1016/j.jneumeth.2009.08.016

Figure 2. Combination of diolistic labeling with immunofluorescence.

Figure 2

A. MSNs in the striatum of triple organotypic cultures were diOlistically labeled with CM-DiI (red) and the TH-fibers visualized by immunofluorescence (green). Areas of apposition between TH-ir afferents and the necks of MSN spines (arrows) were determined using Bitplane software. B. Organotypic triple cultures were labeled with CM-DiI (red) and immunocytochemically stained using anti-VGluT1 antibodies (green). Areas of apposition between MSNs dendritic spine heads and VGluT1-ir terminals were determined using Bitplane software (arrows). A VGluT1-ir presynaptic terminal that appeared close, but did not colocalize with the CM-DiI labeled dendritic spine is indicated by an arrow head. A′,B′, A″, B″. Three-dimensional reconstructions are rendered using Bitplane software. Scale bar = 2.5 μm in A,A′ and B,B′. A″ and B″ show 2.5-fold magnified examples of TH-ir and VGlut1-ir appositions with CM-diI labeled MSNs spines respectively.