Beginning at 14 or 15 days in vitro, hippocampal neurons were cultured in the absence (gray bars) or presence (open bars) of 50 mM ethanol in the tissue culture medium. Average mIPSC amplitude (A), rise time (B), decay time constant (C), and interevent interval (D) are shown for age-matched controls and neurons treated with ethanol for 1 to 5 days. Ethanol treatment did not alter mIPSC amplitude, kinetics, or frequency.