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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Alcohol. 2009 Dec;43(8):603–618. doi: 10.1016/j.alcohol.2009.10.006

Figure 5. Effect of chronic ethanol treatment on the acute effect of benzodiazepine.

Figure 5

Both cortical (A) and hippocampal neurons (B) were cultured in the absence (gray bars) or presence (open bars) of 50 mM ethanol in the tissue culture medium. The increase in mIPSC τd during acute application of 20 μM flurazepam in the external solution is shown. (A) Cultured cortical cells 19-20 days in vitro were less sensitive to flurazepam than control neurons 15-16 days in vitro. Chronic ethanol exposure did not alter mIPSC sensitivity to flurazepam at either timepoint. p < 0.05 ANOVA. * p < 0.05 compared to control 15-16 DIV by Holm-Sidak post-hoc test, † p < 0.05 compared to pre-drug baseline by paired t-test. (B) In cultured hippocampal neurons, flurazepam significantly increased mIPSC τd in control cells 15-16 and 19-20 days in vitro. Ethanol treatment for 1 or 5 days did not alter mIPSC flurazepam sensitivity. † p < 0.05, †† p < 0.01 compared to pre-drug baseline by paired t-test.