Skip to main content
. Author manuscript; available in PMC: 2010 Nov 15.
Published in final edited form as: J Immunol. 2009 Oct 21;183(10):6588–6599. doi: 10.4049/jimmunol.0902029

Figure 7. ES binds and invades HeLa and IEC-6 cells expressing DC-SIGN.

Figure 7

A mammalian expression plasmid containing DC-SIGN cDNA was introduced into HeLa and IEC-6 cells. The transfected cells were examined for the expression of DC-SIGN by flow cytometry using anti-DC-SIGN antibodies (A). In addition, the ability of OmpA+ and OmpA− ES to bind to and invade DC-SIGN expressing HeLa and IEC-6 cells were determined and compared with non-transfected cells (B–E). The error bars represent standard deviations of triplicate samples. The results are representative of three independent experiments. The increase in the binding or invasion of ES was significantly greater compared to control cells, *p<0.001 calculated by Student’s t test.