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. Author manuscript; available in PMC: 2009 Dec 21.
Published in final edited form as: J Mol Cell Cardiol. 2007 Jul 14;43(4):504–516. doi: 10.1016/j.yjmcc.2007.07.001

Fig 3.

Fig 3

Shipped hESC-derived EBs exhibit electric field-stimulated intracellular calcium transients. A: Stacked line scan images depicting electrically evoked calcium-dependent increases in rhod-2 fluorescence in cultured hESC-derived EBs (y-axis, time; x-axis, position). The left panel shows the EFGP epi-fluorescence signal and the middle panel shows the rhod-2 fluorescence signal. The signals were merged in the right panel. B: Spatially integrated traces of the changes in rhod-2 (red) and GFP (green) fluorescence during field stimulation-induced depolarizations in hESC-derived EBs.