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. Author manuscript; available in PMC: 2009 Dec 21.
Published in final edited form as: J Mol Cell Cardiol. 2007 Jul 14;43(4):504–516. doi: 10.1016/j.yjmcc.2007.07.001

Fig 9.

Fig 9

Engrafted hESC-derived cells express connexin 43 at 4 weeks after transplantation into ischemic myocardium. A-C. GFP epi-fluorescence (green; panel A), connexin43 immune reactivity (red, Alexa 555-conjugated secondary antibody; panel B) and a merged image showing both signals (panel C) at the graft/myocardium border. Connexin43 immune reactivity suggestive of the presence of nascent gap junctions can be seen at the junction between a donor and host cells (arrows, panel C; arrowhead indicates a host-host cardiomyocyte junctional complex which is out of the plane of focus). D. Same field depicted in Panels A-C, but focused on the host-host cardiomyocyte junctional complex (arrowhead). E. Phase contrast image of the same field depicted in Panels A-D; note myofibers present in cells interconnected by the junctional complex (arrowhead). F. Connexin43 immune reactivity in remote host myocardium; note the well organized junctional complexes. G. Merged image of GFP epi-fluorescence (green) and connexin43 immune reactivity (red) at the graft/myocardium border; arrow points to putative gap junction. H-I. Merged image GFP epi-fluorescence (green) and connexin43 immune reactivity (red) of donor cells in graft regions with immature (panel H) and more mature (panel I) myofiber structure. Magnification bar correspond to 50 microns.