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. Author manuscript; available in PMC: 2009 Dec 21.
Published in final edited form as: Gynecol Oncol. 2007 Feb 5;105(2):312–320. doi: 10.1016/j.ygyno.2006.12.017

Figure 5. Western analysis of MAP kinase pathway in primary ovarian cancers.

Figure 5

TAK1 (MAP3K7) was detected as an 80 kDa protein (top row). Phosphorylated forms of MKK4 were detected by phosphoserine257/ phosphothreonine262 anti-MKK 4 (2nd row), and phosphoserine 80 MKK4 (3rd row). The total amount of MKK4 protein was detected in row 4. The p38 protein migrated at the expected 38 kDa size (row 5) while total JNK migrated as a doublet of 54 and 46 kDa (6th row). Beta-actin (42 kDa) serves as a loading control (bottom row).