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. Author manuscript; available in PMC: 2009 Dec 21.
Published in final edited form as: Gynecol Oncol. 2007 Feb 5;105(2):312–320. doi: 10.1016/j.ygyno.2006.12.017

Figure 6. EGF induces MKK4 phosphorylation in the SKOV-3 ovarian cancer cell line.

Figure 6

Control SKOV-3 cells were serum starved, and then treated with fresh media without serum (lanes 1–3). Epidermal growth factor (EGF) was added at a concentration of 10ng/ml media (lanes 4–6) for experimental cells. Samples were harvested at 5, 10 and 15 minute time points and subjected to Western blotting. Phosphoserine 80 MKK4 was detected in the top panel, total unphosphorylated MKK4 in the middle panel, and beta-actin in the bottom panel. EGF stimulation of cells induced phosphorylation of MKK4, while the total amount of MKK4 remained constant. After five minutes of EGF stimulation, the fraction of the phosphorylated MKK4 normalized to total MKK4 was 1.7 fold above baseline (lane 1 and 4); at 10 minutes, EGF stimulation increased the phosphorylation by 2 fold (lanes 2 and 5) and at 15 minutes, the phosphorylated isoform of MKK4 was 2.5 fold higher than control (lanes 3 and 6).