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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Biochem Pharmacol. 2009 Sep 24;79(3):487–497. doi: 10.1016/j.bcp.2009.08.031

Figure 8. Effect of EGTA/AM on the action of TCDD.

Figure 8

MMDD1 cells were pre-incubated with 5 μM EGTA/AM for 30 min. (A-C) For mRNA measurement, cells were then treated with 10 nM TCDD for 2 h. The results are expressed as relative mRNA expression levels and the statistically significant differences between control and TCDD treatment are indicated by * (p<0.05) or ** (p<0.01). (D) For western blotting, cells were then treated with 10 nM TCDD for 30 min. The titer of phosphorylated form of the protein kinases in comparison to those of total protein kinases was measured by western blotting.