Table 1.
PCR-RFLP conditions for VDR gene polymorphisms (GenBank AY342401)
| SNP | Primer | Base change | Annealing temperature | Restriction enzyme |
|---|---|---|---|---|
| FokI | 5'AGCTGGCCCTGGCACTGACTCTGCTCT3'(F) | C/T | 61°C | Fok I |
| 5'ATGGAAACACCTTGCTTCTTCTCCCTC 3'(R) | ||||
| BsmI | 5'CAACCAAGACTACAAGTACCGCGTCAGTGA3'(F) | G/A | 57°C | Mva1269 I |
| 5'AACCAGCGGGAAGAGGTCAAGGG3'(R) | ||||
| Tru9I | 5'CAACCAAGACTACAAGTACCGCGTCAGTGA3'(F) | G/A | 57°C | Tru1 I |
| 5'AACCAGCGGGAAGAGGTC AAGGG3'(R) | ||||
| ApaI | 5'CAGAGCATGGACAGGGAGCAA3'(F) | G/T | 60°C | Apa I |
| 5'GCAACTCCTCATGGCTGAGGTCTC3'(R) | ||||
| TaqI | 5'CAGAGCATGGACAGGGAGCAA3'(F) | T/C | 60°C | Taq I |
| 5'GCAACTCCTCATGGCTGAGGTCTC3'(R) |