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. 2009 Jun 16;(28):1308. doi: 10.3791/1308
2X Serum-free Media (SFM)
Final Concentration
(after dilution)
Carnitine 0.099 g 10 mM (5 mM)
Taurine 0.063 g 10 mM (5 mM)
Creatine 0.075 g 10 mM (5 mM)
Antibiotic/Antimycotic 0.5 ml 1% (0.5%) (v/v)
Media 199 45 mL Fill to 50 mL
1X SFM
Final Concentration
2X SFM 25 mL 1X
Media 199 Fill to 50 mL
1X 5% Serum Media
Final Concentration
2X SFM 25 mL 1X
FBS 2.5 mL 5% (v/v)
Media 199 Fill to 50 mL