2X Serum-free Media (SFM) | ||
Final Concentration | ||
(after dilution) | ||
Carnitine | 0.099 g | 10 mM (5 mM) |
Taurine | 0.063 g | 10 mM (5 mM) |
Creatine | 0.075 g | 10 mM (5 mM) |
Antibiotic/Antimycotic | 0.5 ml | 1% (0.5%) (v/v) |
Media 199 | 45 mL | Fill to 50 mL |
1X SFM | ||
Final Concentration | ||
2X SFM | 25 mL | 1X |
Media 199 | Fill to 50 mL | |
1X 5% Serum Media | ||
Final Concentration | ||
2X SFM | 25 mL | 1X |
FBS | 2.5 mL | 5% (v/v) |
Media 199 | Fill to 50 mL |