Table 5.
Average stage-specific detection limit of clinical specimens (n = 3 per species) by QPCR.
| Parasite stage specific limit of detection | |||||||
|---|---|---|---|---|---|---|---|
| rDNA copy number per reaction (parasites per reaction) | |||||||
| Single Rings | Double Rings | Growing Trophozoites | Mature Trophozoites | Gametocytes | Mature Schizonts | Immature Schizonts | |
| P. falciparum | 6.21 ± 2.0 (1.08 ± 0.34) |
1.74 ± 1.09 (0.29 ± 0.18) | 30 (5) |
||||
| P. vivax | 3.66 ± 2.3 (0.60 ± 0.4) |
4.51 ± 0.4 (0.75 ± 0.07) |
21.72 (3.6) |
0.590 (0.098) |
|||
| P. malariae | 5.90 ± 4.29 (0.95 ± 0.75) | 4.74 ± 2.490 (0.78 ± 0.415) | 82.2 (13.7) |
3.03 ± 1.7 (0.5 ± 0.3) |
4.475 ± 1.9 (0.705 ± 0.2) |
4.15 ± 0.94 (0.69 ± 0.16) | |
| P. ovale | 0.56 ± 0.18 (0.08 ± 0.02) | 18 ± 4.5 (3 ± 0.75) |
0.18 (0.03) |
6 (1) |
|||
LOD are stated as either ribosomal DNA copy number or parasites per reaction with standard error where multiple stages were present in specimens.