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. Author manuscript; available in PMC: 2009 Dec 21.
Published in final edited form as: Eur J Immunol. 2008 Aug;38(8):2118–2130. doi: 10.1002/eji.200838213

Figure 2.

Figure 2

Schematic diagram indicating DNA fusion vaccine design. The control vaccine p.DOM contains the sequence encoding the N-terminal domain (DOM) of FrC from TT, including the p30 CD4+ Th epitope. Sequence coordinates relative to the original TT sequence are indicated. p.DOM-gag contains DNA sequence encoding the immunodominant H-2Db-restricted gag85-93 CTL epitope derived from FMuLVgag ligated in-frame to the C terminus of DOM. Each vaccine includes a leader sequence at the N terminus. Vaccine sequences were assembled and inserted into the commercial vector pcDNA3 using Hind III and Not I restriction sites.