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. Author manuscript; available in PMC: 2009 Dec 21.
Published in final edited form as: Eur J Immunol. 2008 Aug;38(8):2118–2130. doi: 10.1002/eji.200838213

Figure 4.

Figure 4

DNA vaccination induces lower-avidity gag85-93-specific CTL in gag-Tg mice compared to WT mice. WT and gag-Tg mice were immunized with p.DOM-gag on days 0 and 28; booster injections at day 28 were administered, together with electroporation. Splenocytes were harvested from individual mice at day 36 and the numbers of SFC secreting IFN- were assessed ex vivo by ELISPOT assay. Splenocytes were incubated with varying concentrations of gag85-93 peptide to assess the epitope-specific CD8+ T cell response; test samples were set up in triplicate for each peptide concentration. Baseline responses without peptide were subtracted and the number of SFC/million splenocytes was calculated as a percentage of the maximum number of SFC/million splenocytes responding per mouse; data were then pooled for each experimental group to calculate the mean ELISPOT response, together with the SEM (error bars). Representative data from one of two identical experiments are shown.