Table I.
Ex vivo detection of vaccine-specific T lymphocytes in wt and gag-Tg mice by IFNγ ELISpot assay.
| Vaccine | Peptide* | Vaccine-specific responses: † | |
|---|---|---|---|
| wt mice | gag-Tg mice | ||
| p.DOM-gag | gag85-93 | 11/11 (100%) | 12/13 (92%) |
| p30 | 10/11 (91%) | 13/13 (100%) | |
| p.DOM | gag85-93 | 0/7 (0%) | 0/8 (0%) |
| p30 | 7/7 (100%) | 6/8 (75%) | |
| Irradiated FBL-3 cells |
gag85-93 | 10/10 (100%) | 0/8 (0%) |
| p30 | 0/10 (0%) | 0/8 (0%) | |
| None | gag85-93 | 0/2 (0%) | 0/3 (0%) |
| p30 | 0/2 (0%) | 0/3 (0%) | |
*
Splenocytes incubated with 0.1μM gag85-93 peptide or 1μM p30 peptide.
†
Data presented as: number of positive responders/total mice tested (% responding).