Table II.
Stimulants used and cytokines measured in the cytokine secretion assays
| Stimulants* | Final Concentration | Cytokines measured with all stimulants | Lower Limit of Detection (pg/ml) |
|---|---|---|---|
| Innate Responses | |||
| Lipopolysaccharide1 | 0.1 μg/mL | IFN-α | 8.2 |
| Polyinosinic-polycytidylic acid2 | 25 μg/mL | IFN-γ | 6.9 |
| Peptidoglycan3 | 1.25 μg/mL | IL-10 | 6.9 |
| CpG-C4 | 1 μg/mL | IL-12p40 | 11.0 |
| Respiratory syncytial virus5 | 500 sfu/mL† | TNF-α | 6.9 |
| Medium alone | -- | IL-8 | 15600.0 |
| Adaptive and Polyclonal Responses | |||
| Phytohemagglutinin6 | 15 μg/mL | IFN-γ | 6.9 |
| Cockroach extract7 | 10 μg/mL | IL-10 | 6.9 |
| Dust mite (D. pteronyssinus) extract8 | 10 μg/mL | IL-13 | 6.9 |
| Tetanus toxoid9 | 10 μg/mL | IL-4 | 6.9 |
| Medium alone | -- | ||
*
Sources for reagents:
1
Associates of Cape Cod (Falmouth, MA),
2
Amersham Biosciences (Piscataway, NJ),
3
InvivoGen (San Diego, CA),
4
Coley Pharmaceuticals (Wellesley, MA),
5
Ann Mosser (University of Wisconsin-Madison),
6
Sigma (St. Louis, MO),
7,8
Greer Inc. (Lenoir NC),
9
Massachusetts Biologics (Jamaica Plain, MA)
†
sfu = syncytium-forming units