Table 3.
Confirmation of bovine segmental duplications by FISH analysis
BAC | FISH | Dup all | Dup intra | Dup inter | Single | No Result | |
---|---|---|---|---|---|---|---|
Both | 33 | 29 | 24 | 22* | 2 | 5 | 4 |
WSSD | 12 | 11 | 8 | 8 | 0 | 3 | 1 |
WGAC < 94% | 1 | 1 | 1 | 1 | 0 | 0 | 0 |
Total | 46 | 41 | 33 | 31 | 2 | 8 | 5 |
Percentage | --- | 100% | 80.49% | 75.61% | 4.88% | 19.51% | --- |
A set of 46 large-insert BAC clones from CHORI-240 library were selected and independent FISH hybridizations were performed. One BAC clone was only supported by WGAC < 94%. FISH signals were categorized as "Single", "Dup intra" or "Dup inter" based on the presence of a single signal or multiple signals on the same or different chromosomes for each probe. *It does not include 154H9, which was scored as both Dup inter and Dup intra.