Primordial follicle formation in mouse fetal ovaries cultured in vitro under various concentrations of E2 in the presence or absence of FSH. A, C, and E) Percentage of single oocytes in the ovaries of each culture group. B, D, and F) Percentage of unassembled oocytes, primordial follicles, and primary follicles in each culture group. G) Total oocytes in the 17.5-dpc mouse fetal ovary after 6 days of culture in vitro. Letters indicate statistically significant differences between treatment groups (P < 0.05).