Table 3. mRNA expression difference in fold change of cytokine and neuronal markers between control and experimental animals.
Marker | mRNA difference between recombinant GM-CSF-injected C57BL6 mice/control mice (PBS injected C57BL6 mice) | mRNA difference between anti-GM-CSF antibody-injected C57BL6 mice/control mice (PBS injected C57BL6 mice) | mRNA difference between recombinant GM-CSF plus antibody-injected C57BL6 mice/control mice (PBS injected C57BL6 mice) |
---|---|---|---|
TNFα | 2.1 | 1.3 | 0.28 |
IL-1β | 0.63 | 0.30 | 0.94 |
IL-6 | 0.36 | 0.35 | 2.89 |
Gp91 | 0.08 | 0.08 | 0.41 |
CD11b | 0.29 | 0.13 | 0.91 |
CD11c | 1.70 | 2.19 | 0.60 |
CD40 | 9.75 | 2.0 | 0.48 |
CD45 | 1.73 | 1.38 | 1.04 |
MHCII -2 | 1.22 | 0.81 | 0.66 |
GFAP | 1.5 | 0.79 | 1.11 |
NeuN | 0.9 | 1.1 | 0.60 |
Note: mRNA fold change was calculated based on comparative cycle threshold (CT) values of real-time RT-PCR between experimental mice/control mice for the following: 1). recombinant GM-CSF-injected C57BL6 mice/control mice (PBS injected C57BL6 mice), 2) anti-GM-CSF antibody-injected C57BL6 mice/control mice (PBS injected C57BL6 mice), and 3). recombinant GM-CSF plus antibody-injected C57BL6 mice/control mice (PBS injected C57BL6 mice).