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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Biotechnol Prog. 2009 Nov;25(6):1810–1818. doi: 10.1002/btpr.208

Table 2.

Monomer Library

graphic file with name nihms121614t1.jpg
A

Nucleic acid sequence of the seven unique Yeast ELP monomer units created using the wobble base system and employed in multimerization of the Yeast ELP gene.

B

Recognition (underlined) and cleavage sites (arrows) for restriction enzymes within the representative monomer, M1, repeat unit.

C

Monomer repeat unit, M1, with cohesive ends generated from a Bbs I / BsmB I sequential digestion and used in the multimerization reaction. Note that Bbs I / BsmB I are type II restriction enzymes and cleave the DNA downstream of their recognition sites.