Figure 3. ERα activation induces phosphorylation of NO signaling proteins.

Activation of NO pathway induced by Provinols™, delphinidin, PPT and 17-β estradiol (E₂) in endothelial cells and consequences of ERα pathway inhibition with either Fulvestrant or siRNA directed against ERα (siRNA). EaHy 926 cells were treated for 10 minutes with Provinols™ (10⁻² g/L), delphinidin (10⁻² g/L), PPT (10⁻⁵ M) or E₂ (10⁻⁵ M) and this in the presence or in the absence of either Fulvestrant (30 nM) (hatched bars) or after knocking down the receptor with siRNA (siRNA) (striped bars). Then cells were lysed to perform Western Blot analysis. *P<0.05, **P<0.01, ***P<0.001 vs non treated; #P<0.05, ##P<0.01 vs Provinols™, delphinidin (A, B, C and D), PPT or E₂ alone (E, F, G and H), (n = 4–6).