Figure 9. Recruitment of myosin-IB at the onset of premature exocytosis.

This cell is expressing GFP-MyoB and mRFP-LimEΔ; it was mixed with unlabeled yeast two hours earlier. The cell is initially migrating (0 time), but the bulky phagosome soon becomes immobilized. At 84 seconds, the immobilized phagosome has brightened (arrowhead), indicating binding of GFP-MyoB, and a vacuole (tailed arrow) is just separating from the phagosome. The vacuole travels across the cell, labeled with GFP-MyoB and trailed by an actin tail (103 and 106 seconds). Perkin-Elmer Ultra View microscope. Bar, 10 µm.