Figure 3.

Live cell fluorescence confocal microscopy. A) Diagramatic version of fusion protein formation. Integrating the fluorescence gene next to the gene of interest in the chosen cell, formation of a fusion protein and visualisation of the protein of interest by the attached fluorescent tag using a microscope. B) Time lapse confocal fluorescence microscopic images of SK-N-AS neuroblastoma cells transfected with a p65-dsred xp expression vector following TNFα stimulation. C) Quantification of protein translocation as nuclear/cytoplasmic fluorescence from the same cell.