Figure 3.

Hybridization analysis of PAGE-1 expression. (A) A multiple tissue dot blot (Left) and Northern blots (Center and Right) were probed with a 140-bp ³²P-labeled PAGE-1 probe under very stringent hybridization conditions (50% formamide, 55°C). Specific PAGE-1 signals were observed in prostate, testis, placenta, and uterus, but not in other tissues (Table 1 legend lists the analyzed tissues). Because the hybridization probe had some similarity with another PAGE-1-like EST cluster that is expressed in testis (PAGE represented by the EST zv62h08, Table 2), we additionally used a probe with minimal homology to zv62h08 to confirm that the signal in testis corresponds to the expression of the authentic PAGE-1. (B) Blots containing 20 μg per lane total RNA from normal or malignant ovary (Right), fallopian tube (Center), and uterus (Left) were hybridized under stringent conditions. PAGE-1 is expressed in fallopian tube, uterus, and uterine cancer, but not in ovary and ovarian cancer.