Table 1.
Comparison of the distribution of PAGE-1 sequences in EST libraries with PAGE-1 Northern hybridization signals
| Tissue | Computational analysis
|
mRNA analysis
|
|||
|---|---|---|---|---|---|
| No. of PAGE-1 ESTs | % PAGE-1/ tissue ESTs | Dot blot | Northern | RTPCR | |
| Prostate | 5 | 5/22,334 (0.022%) | ++ | ++ | + |
| Prostate ca. | 7 | 7/20,871 (0.031%) | + | ||
| Testis | 0 | 0/31,263 | + | + | |
| Testis ca. | 0 | 0/1,123 | + | ||
| Uterus | 3* | 0/22,333 (0.013%) | + | + | |
| Uterus ca. | 0/1,112 | ++ | |||
| Ovary | 0 | 0/5,573 | (+) | − | |
| Ovary ca. | 0 | 0/21,989 | − | ||
| Fallopian tube | 0 | 0/0 | ++ | ||
| Placenta | 8 | 8/49,467 (0.016%) | +++ | +++ | + |
| Other tissues/ca.† | 0 | 0/823,754 | − | − | − |
The human cDNA sequence libraries (dataset of April 25, 1998) were processed by computer analyses as previously described (7). Individual PAGE-1 ESTs are nh24e10.s1, nc27g01.r1, nh24a11.s1, nf19h11.s1, and nr35f03.s1 (prostate); nh32c06.s1, nt72b09.s1, nc33g02.s1/r1, nc79f08.s1/r1, and nt78f01.s1 (prostate cancer); EST81031, EST80996, C18969, C18137, yi82c07.s1/r1, and yw73c12.s1 (placenta); and zr65g11.s1/r1 and aa07e08.s1 (uterus; see ∗ below). % PAGE-1/tissue: the number of PAGE-1 ESTs was divided by the total number of tissue-specific ESTs. Original dot-blot and Northern results are shown in Figure 2. +, ++, and +++ indicate the signal intensity on the dot blots or Northern blots: signal, strong signal, and very strong signal, respectively. Ovary gave a very weak signal after prolonged exposure of the autoradiograph.
The ESTs with uterus specificity were part of pooled uterus-containing libraries; the other tissues of this library did not show and PAGE-1 expression in hybridization analyses.
Other tissues in the database analysis are those that were represented by dbEST files in May 1998. Other tissues that were tested by hybridization analyses were brain, spinal cord, heart, aorta, skeletal muscle, colon, bladder, stomach, pancreas, pituitary, adrenal, thyroid, salivary, mammary, kidney, liver, small intestine, spleen, thymus, peripheral leukocyte, lymph node, bone marrow, appendix, lung, trachea, fetal brain, fetal heart, fetal kidney, fetal liver, fetal spleen, fetal thymus, fetal lung, and cancer cell lines HL60, HeLa, K562, Molt4, Raji, SW480, A549, and G361.