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. Author manuscript; available in PMC: 2010 Apr 30.
Published in final edited form as: Cell. 2009 Oct 30;139(3):485–498. doi: 10.1016/j.cell.2009.09.033

Figure 4. SFB induce Th17 cell differentiation upon colonization of Jackson C57BL/6 mice.

Figure 4

A. Colonization of the terminal ileum by SFB 10 days after transfer of fecal homogenates from SFB-mono mice into Jackson B6 mice

B-C. IL-17 and IL-22 expression in TCRβ+CD4+ SI LPL in Jackson B6 mice colonized with SFB (Jax+SFB) compared to controls (Jax). Data from one of two experiments

D-E. Jackson microbiota induces Th17 cells only when complemented with SFB. Germ-free IQI mice were colonized with SFB, Jackson microbiota isolated from fecal pellets by itself (Jax), or a mixture of both (Jax + SFB). Th17 cell proportions were analyzed in the LP 3 weeks later by flow cytometry. Plots in (D) gated on total lymphocytes. Data in (E) represent percentage of IL-17+ cells in the CD4+ gate

F. RT-PCR for Th17 cell effector cytokines in total RNA from terminal ileum of the mice in (E)