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. Author manuscript; available in PMC: 2010 Apr 30.
Published in final edited form as: Cell. 2009 Oct 30;139(3):485–498. doi: 10.1016/j.cell.2009.09.033

Figure 6. SFB colonization induces SAA expression that influences Th17 differentiation.

Figure 6

A. Relative mRNA expression levels of SAA1-3 genes by real-time RT-PCR in the terminal ileum of IQI GF mice (GF) colonized with fecal homogenates from SFB-mono mice (SFB), Jackson B6 mice (Jackson), or a mixture of both (Jackson+SFB).

B. Splenic naïve CD4+ T cells were cocultured with or without LP CD11c+ cells in the presence of an anti-CD3 antibody with the indicated concentration of recombinant Apo-SAA for 4 days. T cells were collected, restimulated with PMA and ionomycin, and real-time RT-PCR performed. Results were normalized to expression of GAPDH mRNA. The data are representative of four independent experiments with similar results.