Table 1.

Summary of CPP and ACPP acute toxicity in mice. Nude mice were IV injected with peptide and observed for acute toxicity resulting in rapid death. Mouse survival of immediate period after injection was recorded, each showing no signs of moribundity or significant weight loss for at least 2 days post injection. Unlabeled peptide injected, dose injected IV, range of mice mass, time of death, and number survived (minimal 24 hours with no signs of toxicity) are listed in the table.

Peptide	Dose	Mouse Mass (range)	Time of death	Survived
r9 CPP	2.5 µmol/kg (53–65 nmols)	21.7–26.0g	15–20 min n=1	4/5
	5 µmol/kg (105–117 nmols)	21.0–25.7g	<3 min n=5	0/5
PLGLAG cleavable ACPP	10 µmol/kg (220–266 nmols)	22.3–26.6g	NA	5/5
	20 µmol/kg (352–480 nmols)	16.0–24.0g	10–20 min n=5	0/5
(PEG2)2 uncleavable ACPP	10 µmol/kg (182, 202 nmols)	20.6–23.3g	NA	2/2
	20 µmol/kg (405 nmols)	20.2–21.2g	NA	2/2
	50 µmol/kg (1,100–1,280 nmols)	22.0–25.6g	NA	3/3