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. Author manuscript; available in PMC: 2009 Dec 22.
Published in final edited form as: J Immunol. 2008 Apr 1;180(7):4487–4494. doi: 10.4049/jimmunol.180.7.4487

Figure 6. The epithelial antigen recognized by BOS+ serum reactivity is K-α1 tubulin protein.

Figure 6

Purified recombinant K-α1 tubulin protein was electrophoresed and transferred onto NCP and probed with BOS+ sera and BOS- sera. The figure shows a representative BOS+ serum with epithelial reactivity binding to the purified K-α1 tubulin protein whereas no reactivity was observed with BOS- sera (A). In order to further confirm, two strips with the purified recombinant K-α1 tubulin protein were blocked with anti-K-α1 tubulin antibody (+) or control antibody (-) and probed with BOS sera with AEC reactivity. Blocking with specific antibody resulted in abolition of BOS sera reactivity with epithelial cells (B).