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. Author manuscript; available in PMC: 2009 Dec 22.
Published in final edited form as: J Pharmacol Exp Ther. 2007 Nov 21;324(2):484–496. doi: 10.1124/jpet.107.129890

Fig. 2.

Fig. 2

Deoxy analogs retain high affinity only for GluR5 subunits. A, displacement of [3H]kainate and [3H]AMPA from KA and AMPARs, respectively, for 8-deoxy-neoDH (left) and 9-deoxy-neoDH (right). Glutamate (1 mM) was used to determine nonspecific binding. Curves were fit with a one-site competition curve with fixed minima (0%) and maxima (100%). n = 3 to 5 for each concentration of analog on each receptor subunit. Ki values were calculated with the Cheng-Prusoff equation using the determined IC50 values and the radioligand Kd value (Table 1). B, both deoxy analogs are GluR5 agonists. Traces are representative of single control responses after 100-ms application of saturating concentrations of glutamate (10 mM) to GluR5-2a-expressing cells and analog-evoked currents during a 1-s application for 100 μM 8-deoxy-neoDH (left) and 100 μM 9-deoxy-neoDH (right).