Table 1.
Solution | Component (mM) |
||||||||
---|---|---|---|---|---|---|---|---|---|
Na+ | Ca2+ | Cl− | OH− | HEPES− | Cs+ | F− | Sucrose | ||
External | LACSF | 155.95 | 2 | 154 | 5.95 | 4.05a | – | – | 54 |
HACSF | 156 | 20 | 190 | 6 | 4a | – | – | – | |
Internal | – | – | 90 | 3.5 | 6.5a | 183.5 | 90 | – |
aThe total concentration of HEPES added to solutions was 10 mM. These values are estimates of HEPES concentrations after balancing the pH to 7.38 by NaOH and CsOH for external and internal solutions, respectively.