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. 2009 Oct 20;19(2):250–261. doi: 10.1093/hmg/ddp484

Figure 6.

Figure 6.

Chx10/Vsx2 acts with other TFs to synergistically activate the Nxnl1 promoter. Activity of the Chx10/Vsx2 with Vsx1, Pax4, TLX2, Usf1, Sp3 and Yy1 TFs on the (A) mouse and (B) on human Nxnl1 promoter. Activity tested by transient transfection in HEK 293 cells with dual luciferase assay. About 0.5 µg of each TF was tested individually or paired with 0.5 µg of Chx10/Vsx2 in co-transfection assays with 0.1 µg of reporter plasmid. The results are expressed as percentage of synergistic activation over the sum of both TFs when tested individually. The results were normalized by internal control (Renilla) driven by the thymidine kinase promoter. Each experiment was repeated in triplicate.