Figure 4.

Bioenergetic variations were preserved after ‘re-cybridization’. (A) BN-PAGE of RC complexes from cybrids obtained by ‘re-cybridization’. (B) Western blot of mitochondrial proteins from cybrids obtained by ‘re-cybridization’ solubilized as in (A), resolved by denaturing SDS–PAGE, and detected with specific antibodies. (C) ATP synthesis in cybrids obtained by ‘re-cybridization’ expressed as a percentage of 143B cells. (D) Coupled respiration (top) and ATP synthesis (bottom) of ATP6 mutant cybrids obtained by ‘re-cybridization’ in a d407 retinal pigmentary epithelium background expressed as a percentage of d407 wild-type control cells. In (C) and (D), values are average of at least three independent measurements. *P < 0.05, **P < 0.005 versus wild-type cells.