Skip to main content
. 2009 Dec 22;106(52):22223–22228. doi: 10.1073/pnas.0912250106

Fig. 4.

Fig. 4.

msh2 and msh6 domain II mutants have MMR defects in vivo. (A) Residues mutated in S. cerevisiae Msh2 in the same region as the E. coli mutS-211 mutations (PDB ID code 1e3m, green) are colored in red on the model of H. sapiens Msh2 domain II (PDB ID code 2o8b, violet). (B) MSH2 complementation of a msh2Δ strain. The indicated msh2 alleles were expressed on a low-copy-number plasmid bearing a marker allowing growth on media lacking Ura. Plasmids were transformed into a msh2Δ strain, and isolates were patched onto plates lacking Ura and then replica plated onto plates lacking Ura and Lys or Ura and Thr. (C) Residues mutated in S. cerevisiae Msh6 in the same region as the E. coli mutS-211 mutations (PDB ID code 1e3m, green) are colored red on the model of H. sapiens Msh2 domain II (PDB ID code 2o8b, blue). (D) MSH6 complementation of a msh3Δ msh6Δ strain. The indicated msh6 alleles were expressed on a low-copy-number plasmid bearing a marker allowing growth on media lacking Leu. Plasmids were transformed into a msh3Δ msh6Δ strain, and isolates were patched onto plates lacking Leu and then replica plated onto plates lacking Leu and Lys or Leu and Thr.