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. Author manuscript; available in PMC: 2009 Dec 22.
Published in final edited form as: Biochemistry. 2006 May 9;45(18):5800–5816. doi: 10.1021/bi060217r

Fig. 3.

Fig. 3

Analysis of the OS2 mutants and chimeras by SDS-PAGE electrophoresis and CD. (A) One µg of sPLA2 was loaded on a 14% SDS-polyacrylamide gel under reducing conditions and stained with Coomassie brilliant blue. The apparent mobility appeared to be very sensitive to the pI of the protein (B) CD spectra of recombinant WT OS2, H48Q mutant and C-terminal chimera compared to that of venom OS2. The CD spectra of all the other mutants are identical to those shown here.