. Author manuscript; available in PMC: 2009 Dec 22.

Published in final edited form as: Biochemistry. 2006 May 9;45(18):5800–5816. doi: 10.1021/bi060217r

TABLE 1. Recombinant production of OS₂ and its mutants.

All proteins were produced using the pAB3 E.coli expression system. The yield of production is the amount of pure protein obtained per liter of E. coli culture. The proteins were analyzed by MALDI-TOF mass spectrometry to confirm that all the disulfide bonds are formed and that proteins have not been covalently modified during purification.

sPLA₂	Yield of production	Calculated mass	Measured mass	Δmass
	mg/liter	Dalton	Dalton
OS₂ WT	5	13317.08	13317.86	0.78
OS₂ H48Q	2.6	13308.07	13307.27	0.80
OS₂ D49K	6.4	13330.17	13329.84	0.33
OS₂ G30S	1.3	13347.11	13347.89	0.78
OS₂ K31L-R34S	1.5	13232.96	13233.79	0.83
N-terminal chimera	0.3	13278.02	13277.30	0.72
Central chimera	1	13992.66	13993.29	0.63
C-terminal chimera	2.6	13429.10	13430.12	1.02

TABLE 1. Recombinant production of OS2 and its mutants.

TABLE 1. Recombinant production of OS₂ and its mutants.