Figure 2.

Dual viral tracing using genetically modified virus. (A) Neurons which have a divergent projection to two brain regions will be infected by retrograde transsynaptic transport of the two recombinant viruses, and will be double-labeled by the co-expression of the two marker proteins. (B) Two RV recombinants were generated for dual viral tracing by inserting different marker genes encoding either β-gal or EGFP. (C) Neuron double-labeled by β-gal (magenta, left panel) and EGFP (green, center panel) can be seen in white in the merged image (right panel). Scale bar = 20 μm.