Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Dec 15;3(3):344–349. doi: 10.3389/neuro.01.032.2009

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2009 Ohara, Inoue, Witter and Iijima.

This is an open-access article subject to an exclusive license agreement between the authors and the Frontiers Research Foundation, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are credited.

PMC Copyright notice

Experimental design of dual viral tracing in the rat entorhinal-hippocampal circuit. (A) rHEP5.0-CVSG- β-gal and rHEP5.0-CVSG-EGFPx2 were injected to the left and right MEC, respectively. The viruses, transsynaptically transported from the bilateral MEC will go through CA1 and will first meet in the hippocampal CA3 region. (B) In field CA1, neurons are labeled only by the virus (rHEP5.0-CVSG- β-gal) injected into the ipsilateral MEC, whereas β-gal (magenta) and GFP (green) labels intermingle in CA3 region. Note that there are double-labeled neurons (white) in CA3. MEC, medial entorhinal cortex. Scale bar = 500 μm.