Fig. 8.
Misexpression of atf3 enhances the effect of Rho1 silencing and leads to mislocalization of the Rho1 protein. (A-C) Rho1 RNAi alone (A,B) caused abdominal defects similar in extent to those in ppl>atf3 animals (see Fig. 4A,B), whereas combined with misexpression of atf3 (C) it led to broader, lethal scars (compare arrows). (D) The effect of ectopic atf3 was averted by co-expression of active Rho1V14. All transgenes were induced under the ppl-Gal4 driver. (E,E′) Rho1 is redistributed in GFP-marked LECs (28 hours APF) that express atf3 upon flp-out induction. DAPI (magenta) is used for staining nuclei. Scale bar: 50 μm.