Fig. 1.

Mutations in GRAND CENTRAL (GCT) and CENTER CITY (CCT) cause decreased expression of the peripheral-abaxial enhancer trap E2023. (A-F) E2023 is a KAN2 reporter expressed in the peripheral-abaxial domain of wild-type plants. Early heart (A), late heart (B), torpedo (C) and bent cotyledon (D) embryos; inset in D shows embryo cross section. Abaxial view of a 10-day-old seedling (E) reveals that E2023 is expressed in abaxial vascular tissue of expanded cotyledons and leaves, and throughout the abaxial side of leaf primorida (arrowhead). Carpels of wild-type flower (F). gct (G-L) and cct (M-R) delay and reduce the expression of E2023. Early heart (G,M), late heart (H,N), torpedo (I,O) and bent cotyledon (J,P) stage embryos. Ten-day-old gct (K) and cct (Q) seedlings (insets in K and Q: 2× magnification of leaves). gct (L) and cct (R) carpels. Scale bars: 20 μm in A-D,G-J,M-P; 1 mm in E-F,K-L,Q-R.