Fig. 4.

GCT and CCT encode the Arabidopsis homologs of the transcriptional regulators MED13 and MED12, and are dynamically expressed during embryogenesis. (A) GCT corresponds to At1g55325. The location and the nature of mutant alleles are shown. Serine-rich region (AA 407-426) shown in dark gray. Region of 42% identity (P-value 9e⁻¹¹) to pfam06333 MED13 domain shown in light gray (AA 1088-1179). (B) CCT corresponds to At4g00450. Region of 37% identity (P-value 5e⁻¹³) to pfam09497 MED12 domain (AA 69-127) is shown in light gray. (C-K) mRNA in situ hybridization analysis of GCT expression in (C) one-cell, (D) octant, (E) dermatogen, (F) globular, (G) heart, (H) torpedo, (I) bent cotyledon, (J) SAM of bent cotyledon, and (K) RAM of bent cotyledon stage embryos. (L-R) RNA in situ hybridization analysis of CCT expression in (L) dermatogen, (M) globular, (N) heart, (O) torpedo, (P) bent cotyledon, (Q) SAM of bent cotyledon and (R) RAM of bent cotyledon stage embryos. (S) GCT sense probe; (T) CCT sense probe. Scale bar: 10 μm.