Fig. 2.

Cytometric bead array (CBA) analysis of Th1/Th2 cytokine production by PBMCs from SARS-recovered individuals in response to S, M, E and N peptides. PBMCs from five SARS-recovered donors (patient 1, 2, 5, 12 and 20) were seeded in 96-well plates with or without S, M, E and N peptides for 72 h. The culture supernatants were collected, and Th1/Th2 cytokine production was measured serially by CBA using a FACSCalibur flow cytometer. The data were generated in graphical format using the BD CBA software. The assay sensitivity with these five cytokines was 20 pg/ml. a S peptides, b M peptides, c E peptides, d N peptides