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. Author manuscript; available in PMC: 2010 Mar 15.
Published in final edited form as: J Immunol. 2009 Mar 15;182(6):3583–3596. doi: 10.4049/jimmunol.0802533

Figure 5.

Figure 5

Marginal zone (MZ) splenic cells in 56RVκ8 mice include H and L chain edited B cells. A. Flow cytometric dot plots of CD21 versus CD23 for B220 gated lymphocytes showing presence of MZ splenic B cells (CD21+CD23−) in 56RVκ8 mice and the non-transgenic (BL/6 × BALB/c) F1 control, but not in 3H9Vκ8 or 56RVκ8 scid/scid (s/s) mice. B. Flow cytometric dot plots of IgMa versus IgMb and IgMa versus Vλx for B220 gated lymphocytes of 56RVκ8 mice. Lower dot plots show CD21/CD23 staining profiles for cells expressing sIgMb, Vκ21D (corresponding to sIgMa/sIgMb doubly stained cells (32)) and for cells expressing low (lo) and high (hi) surface Vλx (Vλxlo and Vλxhi). Numbers within and outside the boxed areas in A and B indicate the percentage of cells with the indicated phenotype.