Table 1.

Primers and probes used in the identification of Anopheles funestus species using real time assays (MCA, HRM and TaqMan)

Name	Primer /probe	Reporter Dye	Sequence (5'-3')	Quencher
FUN1	Primer	-	GGCATCGATGGGTTAATCATG	-
VAN1	Primer	-	AAACCCCAAGATGTGCTCC	-
VAN3	primer	-	GGTTTTCAAATGAATCTC	-
N PAR	Primer	-	ATACTTGTGTGTGTGTGTATTTG	-
RIV TM	probe	Cy5	CTATGGCGAGACCCCGTCTAGTG	BHQ2a
FUN TM	probe	ROX	CATGGGGAAATTCAATCGAAAACCTCT	BHQ2a
PAR TM	probe	VIC	CGG AAC CTA GCT TGG	MGBNFQb
VAN TM	probe	Quasar 705	CGT TGT GAA AAA TGG AGA TTC ATT TGA AAA CC	BHQ2a
LEES TM	probe	6-FAM	CCG ACC GAT GTA CA	MGBNFQb

^aBHQ2 (black hole quencher 2)

^bMGBNFQ (minor groove binder non-fluorescent quencher)